![]() Two BMP-like molecules expressed in somatic cells, decapentaplegic (Dpp) and glass bottom boat (Gbb), are required for GSC maintenance and repress the differentiation factor bag-of-marbles (Bam) by bone morphogenetic protein (BMP) signaling 10. Hh activates the Hh signaling pathway in CySCs, and is required for the maintenance of CySCs 9. Upd binds with Domeless (Dome) and activates the Janus kinase/signal transducer and the activator transcription (JAK/STAT) pathway in both GSCs and CySCs, and maintains their self-renewal ability 7, 8. Hub cells secrete unpaired (Upd) and hedgehog (Hh) proteins. ![]() CySCs provide the environment necessary to trigger GSC differentiation by the non-cell-autonomous approach 6.Įarly germ cells have been shown to be tightly controlled by niche signaling. Somatic cells, including apical hubs and CySCs, form the stem cell environment for neighboring GSCs, and CySCs have been proposed to be a source of instructive self-renewal signals 5. The gonialblast undergoes four rounds of transit-amplifying (TA) spermatogonial divisions to generate a 16-cell spermatogonia cluster in which individual germ cells are connected by ring canals and a branched fusome 4. In Drosophila testes, GSCs asymmetrically divide to generate one cell that retains stemness and a gonialblast that proliferates and differentiates 2. The stem cell niche, a key microenvironment that regulates stem cell behaviors, supports two distinct adult stem cell populations: germline stem cells (GSCs) and cyst stem cells (CySCs) 1, 2, 3. Stem cells are undifferentiated populations with the remarkable potential of self-renewal and differentiation. This study provides new insights for elucidating the pathogenic mechanism of male sterility and the formation of testicular germ cell tumor. Collectively, our findings uncover the genetic causes and molecular mechanisms underlying the stem cell niche. Srlp also regulates the expression of spliceosome and ribosome subunits and controls spliceosome and ribosome function via RpL6 signals. Furthermore, results of the liquid chromatography-tandem mass spectrometry (LC-MS/MS) reveal that RpL6 binds to Srlp. In the in vitro assay, Srlp is found to control the proliferation ability and cell death in S2 cells, which is consistent with the phenotype observed in Drosophila testis. In Drosophila, Srlp is an essential gene that regulates the self-renewal and differentiation of GSCs in the testis. Here, using genetic manipulation of the Drosophila model, we systematically analyze the function and mechanism of Srlp in vivo and in vitro. However, the underlying mechanistic links between Srlp and the stem cell niche remain largely undetermined. In our previous study, we screened the novel GSC regulatory gene Srlp in Drosophila testes. Self-renewal and differentiation in germline stem cells (GSCs) are tightly regulated by the stem cell niche and via multiple approaches.
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